HP1/ORC complex and heterochromatin assembly.

TitleHP1/ORC complex and heterochromatin assembly.
Publication TypeJournal Article
Year of Publication2003
JournalGenetica
Volume117
Issue2-3
Pagination127-34
ISSN0016-6707
Abstract

We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylatedisoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed.

Short TitleGenetica
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