Biology Imaging Facility

Confocal Microscopy
Conventional Microscopy


Confocal Microscopy

A Leica TCS confocal microscope is available for use by members of the Biology Department. The Leica software allows image manipulations for 3-D reconstruction of optical sections, creation of movies showing rotation of images, and calculations of intensities and ratios for techniques such as FRET and FRAP.

Currently available features include:

  • Leica DMRBE research microscope (upright configuration)
  • Leica DMIRBE inverted microscope (inverted configuration)
  • Leica TCS SP spectral detection head
  • 10x-100x objectives, plus long working distance 40x & 63x water immersion objectives
  • Four lasers for excitation of fluorochromes from UV to far red:
    351 nm, 458 nm, 488 nm, 561 nm, 633 nm

All users of the Biology Department confocal microscope must receive training prior to use. Please contact Dr. Harrison for more information.

Usage protocols:
There are basic protocols for the use of the Leica confocal microscope.  In addition, periodic "Confocal Notes" are distributed by Leica to provide "how-to" guides for common issues and applications.
 

Conventional Microscopy

  • Nikon E800 Epifluorescence Compound Microscope
  • Objectives from 4x to 100x magnification
  • Differential Interference Contrast (DIC/Nomarski) optics for 10x to 100x objectives
  • Fluorescence filter sets appropriate for DAPI, FITC/GFP, RITC/TRITC and related fluorochromes
  • Nikon SMZ1500 Epifluorescence Stereomicroscope
  • Fluorescence filter sets  for DAPI, BFP, CFP, YFP, FITC/GFP, and RITC/DsRed
  • Conventional microscopes use a Spot RT Slider digital camera to capture light or fluorescence micrographs.
     

All microscopes are housed in T.H. Morgan Room 300A and are available for use by Biology Department researchers on a first-come first-served basis.  Questions about microscopy resources should be directed to Dr. Harrison.

 

 

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