Olfactory receptor surface expression is driven by association with the beta2-adrenergic receptor.

TitleOlfactory receptor surface expression is driven by association with the beta2-adrenergic receptor.
Publication TypeJournal Article
Year of Publication2004
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue37
Pagination13672-6
Date Published2004
ISSN0027-8424
Abstract

Olfactory receptors (ORs) comprise more than half of the large class I G protein-coupled receptor (GPCR) superfamily. Although cloned over a decade ago, little is known about their properties because wild-type ORs do not efficiently reach the cell surface following heterologous expression. Receptor-receptor interactions strongly influence surface trafficking of other GPCRs, and we examined whether a similar mechanism might be involved in OR surface expression. Olfactory neurons are known to express beta-adrenergic receptors (ARs), and we found that coexpression with beta(2)-ARs, but not any other AR subtypes, dramatically increased mouse 71 (M71) OR surface expression in human embryonic kidney 293 cells. A persistent physical interaction between M71 ORs and beta(2)-ARs was shown by coimmunoprecipitation and by cointernalization of the two receptors in response to their specific ligands. Also, coexpression of wild-type M71 ORs with beta(2)-ARs resulted in cAMP responses to the M71 ligand acetophenone. Finally, in situ hybridization studies showed extensive colocalization of M71 OR and beta(2)-AR expression in mouse olfactory epithelium. These data demonstrate the successful heterologous surface expression of a functional wild-type OR and reveal that persistent physical association with other GPCRs can control OR surface expression.

URLhttp://www.pnas.org/cgi/pmidlookup?view=long&pmid=15347813
DOI10.1073/pnas.0403854101
Short TitleProc Natl Acad Sci U S A
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