Stable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint.

TitleStable hZW10 kinetochore residency, mediated by hZwint-1 interaction, is essential for the mitotic checkpoint.
Publication TypeJournal Article
Year of Publication2008
JournalThe Journal of cell biology
Volume180
Issue3
Pagination507-20
Date Published2008
ISSN0021-9525
Abstract

The mitotic checkpoint is an essential surveillance mechanism that ensures high fidelity chromosome segregation during mitosis. Mitotic checkpoint function depends on numerous kinetochore proteins, including ZW10, ROD, and Zwilch (the ROD-ZW10-Zwilch complex). Through an extensive mutagenesis screen of hZW10, we have mapped the kinetochore localization domain of hZW10 as well as the hZwint-1 interaction domain. We find that hZwint-1-noninteracting mutants still localize to kinetochores. In addition, using fluorescence recovery after photobleaching, we have found that hZW10 residency at metaphase kinetochores is brief (half-time of 13 s). However, during prometaphase or at unattached kinetochores, enhanced green fluorescent protein-hZW10 becomes a stable component of the kinetochore. Moreover, we find that stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest.

URLhttp://jcb.rupress.org/cgi/pmidlookup?view=long&pmid=18268100
DOI10.1083/jcb.200708021
Short TitleJ Cell Biol
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